Enumeration of mesophilic lactic acid bacteria at 30C


ISO 15214:1998

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ISO 15214:1998 This International Standard specifies a horizontal method for the enumeration of viable mesophilic lactic acid bacteria by counting the colonies growing in a solid medium after incubation at 30 ?C for 3 days. NOTE In some food products, there exist psychotrophic or thermophilic lactic acid bacteria necessitating culture temperatures different from 30 ?C. Moreover, not all lactic acid bacteria grow on MRS agar at pH 5,7 and some grow only weakly.